av M Lindåse · 2007 — MHC (B) genes and the gene products from the class I and II genes are of ng genomic DNA, 1x PCR buffer II (Applied Biosystems, Foster City, CA, USA), 2.5.

The purchase of these products is accompanied by a limited license to use them in the Polymerase Chain Reaction (PCR) process in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front fee, either by payment to Applied Biosystems or as purchased, i.e. an authorized thermal cycler.

HiScript® II One Step RT-PCR Kit is specially designed for end-point PCR detection using RNA as template (such as RNA virus). Using gene-specific primers (GSP), reverse transcription and PCR reactions are completed in one tube, no additional tube opening/pipetting operations are required, which greatly improves detection throughput and reduces the risk of contamination. HiScript II 1st Strand cDNA Synthesis Kit-Vazyme Biotech Co.,Ltd- HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology on the basis of M-MLV (RNase H-) Reverse Transcriptase. HiScript II Q Select RT SuperMix for qPCR-Vazyme Biotech Co.,Ltd- HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology on the basis of M-MLV (RNase H-) Reverse Transcriptase. Applied Biosystems™ AmpliTaq Gold™ Fast PCR Master Mix. Premix, hot start Applied Biosystems™ GeneAmp™ 10X PCR Buffer II & MgCl2. Optimized for  PCR Buffer II (100 mM Tris-HCl, pH 8.3, 500 mM KCl) 10X .………. Applied Biosystems®, catalog number N808-0010.

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Isothermal Amplification Buffer II Pack Isothermal Amplification Buffer Pack Magnesium Chloride (MgCl 2) Solution Magnesium Sulfate (MgSO 4) Solution Nuclease P1 An optimal buffer system is essential to perform successful PCR. Reliable PCR results depend on many factors: the quality of the DNA and primers as well as the PCR instrument itself. Ampliqon has developed different Tris-based buffer solutions to meet different requirements in PCR applications. The buffers are commonly supplied in 10x formulations GeneAmp® 10X PCR Buffer II and MgCl 2, 2 x 75 mL P/N: 4379878 Buffer Characteristics GeneAmp 10X PCR Buffer II provides preferred pH and ionic strength for PCR amplification reactions. The magnesium ion concentration required to achieve optimal PCR amplification is dependent on the specific set of primers and template used. A 25 mM MgCI PCR is a versatile and robust method for the rapid qualitative and quantitative amplification of target DNA. Real-time PCR is more precise in quantifying nucleic acid than conventional PCR, because, with real-time PCR, the amplified nucleic acid is detected in the exponential rather than the plateau phase with conven-tional PCR. The PCR Buffer is supplied as a 10X concentrate and should be diluted 1:10 in the final reaction (e.g., use 5 µl in a 50-µl PCR reaction).

av G Canesin · 2017 · Citerat av 38 — Prostate cancer is the second most frequently diagnosed cancer in men and it RNA extraction, reverse transcriptase PCR and real-time PCR Briefly, 40 μg of total proteins were prepared in 4x Laemmli buffer and heated to (a-b) MTT viability assays on DU145 (a) and DU145-Luc cells (b) after 24 h of 

bioWORLD. Optimized PCR buffer set for amplification of specific DNA fragments in conventional PCRs ( i.e ., with Taq DNA polymerase).

Description. Applied Biosystems® GeneAmp® 10X PCR Buffer I is optimized for use with AmpliTaq® DNA Polymerase, resulting in robust PCR amplification. • Optimized for use with AmpliTaq® DNA Polymerase, offering maximum PCR performance. • Available in several convenient sizes. GeneAmp® 10X PCR Buffer I contains 15 mM MgCl 2.

Our mission is to develop high-quality innovative tools and services to accelerate discovery. The purchase of these products is accompanied by a limited license to use them in the Polymerase Chain Reaction (PCR) process in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front fee, either by payment to Applied Biosystems or as purchased, i.e.

CloneID 1X COLONY PCR MASTER MIX . Lucigen. Finns i flera varianter. GoTaq G2 Green Master Mix .
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ZERO BIAS - … Description. Invitrogen Platinum II Green PCR Buffer is an innovative buffer optimized for use with Platinum II Taq Hot-Start DNA Polymerase.

All buffers contain Tris and 15 mM MgCl₂ (1,5 mM MgCl₂ final concentration). Additional MgCl₂ for easy optimisation is included in a separate vial. Ab Buffer Kit Introduction Ab Buffer Kit contains 10X stock solutions of Binding and Elution buffers and ready to use Neutralizing buffer. The buffers are optimized for rapid purification of monoclonal and polyclonal IgG using immobilized Protein A or Protein G. The kit eliminates time-consuming buffer preparation and thus PCR Buffer Set, 1.25 mL.
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10X PCR Buffer: 100 mM KCl, 20 mM MgSO4·7H2O, 200 mM Tris-HCl, pH8.8, 1.0% Triton X-100, 100 mM (NH4)2SO4, 1 mg/ml BSA PCR. Advantages • Ready-to-use mix for fast PCR set up • Premixed with DNA loading dye allowing for quick agarose gel loading • 100 rxns (amplification of long targets: 100 bp – …

Manufacturer: Applied Biosystems™ N8080130. View more versions of this product. The enzyme's half-life is ~40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. This AmpliTaqDNA Polymerase is supplied with GeneAmp 10X PCR Buffer II and MgCl 2 Solution. For superior PCR performance, we … • Includes GeneAmp® 10X PCR Buffer II with MgCl 2 Hot-start activation The modified enzyme is provided in an inactive state.

Ta ut 10 µM-primrar, 10 mM dNTP:eroch DNA Polymerase 10X PCR Buffer (PN 703315) ur frysen och Det är viktigt att separera (i) DNA-isolering; (ii) PCR-.

Compare Products: Select up to 4 products. *Please select more than one item to compare 2011-12-02 · One unit of AccuPrime Taq DNA polymerase High Fidelity (Invitrogen) was used in 50 μl 1× AccuPrime PCR buffer II. The thermoprofile of AccuPrime Taq HiFi reactions included the same long denaturation steps as the 'long denaturation' Phusion protocol above: 3 minutes at 98°C; 10 × (80 s at 98°C, 90 s at 65°C or 60°C); 10 minutes at 65°C or 60°C. QIAGEN's fully integrated nanoplate-based digital PCR system, has been designed with your research needs and the limitations of the currently available methods in mind. It's time to simplify the transition from qPCR and prepare your critical research applications for the changing landscape of digital PCR. setting up PCR reactions. B) Setting up PCR reactions Negative Controls Use two negative controls among the PCRs.

Optimized PCR buffer set for amplification of specific DNA fragments in conventional PCRs ( i.e ., with Taq DNA polymerase). The PCR Buffer Set includes: 2 x 1.25ml each of 10X Mg2+ PCR free buffer (100mM Tris-HCl, KCL (pH 8.3) 2 x 1.25ml of 25mM MgCl 2. Compare this item. ABScript II One Step RT-qPCR Probe Kit a ready-to-use kit allowing reverse transcription and subsequent probe-based qPCR in a single tube. It contains all components for RT-qPCR except primers, probes and RNA templates. The one-step format significantly improves sensitivity and effectively prevent contamination. Ab Buffer Kit Introduction Ab Buffer Kit contains 10X stock solutions of Binding and Elution buffers and ready to use Neutralizing buffer.